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Edição atual tal como às 08h25min de 15 de fevereiro de 2020

The virus titers in (E) kidney, (F) liver, (G) lung, (H) mesenteric lymph node and (I) brain were determined on day 10 post infection. (J) IL-10-/- mice were intravenously infected AP24534 mw with 106 pfu LCMV Docile, 106 pfu LCMV Clone 13 or a mixture of 5��105 pfu LCMV Docile and 5��105 pfu LCMV Clone 13. Virus titers in the spleen were analyzed on day 10 post infection. Each symbol represents an individual mouse. The solid line indicates the mean value and the dashed line the detection limit. (A-D) Data from one representative experiment of two is shown. (E-I) Data from two pooled experiments are depicted. Significance was determined by unpaired 2-tailed Student's t test. * P Emricasan CD3+ cells. (J) B cells were pregated on CD19+ and B220+ cells. (K) T cells were gated on CD3+ cells. The numbers indicate the means �� SD of three mice per group. One representative of two experiments is shown. ""Thirty-three cefazolin-resistant extraintestinal pathogenic Escherichia coli strains from companion animals were screened for blaCMY-1, blaCMY-2, blaSHV, blaTEM, and blaCTX-M genes. Extended-spectrum Rapamycin ��-lactamase-producing strains were further characterized by O serotyping and multilocus sequence typing. It was found that 20 and 17 isolates harbored TEM-1 and CMY-2 ��-lactamases, respectively, and 13 isolates harbored both ��-lactamases. One isolate harbored DHA-1 ��-lactamase. Eleven isolates were found to possess CTX-M ��-lactamases (CTX-M-27 [n= 6], CTX-M-14 [n= 3], CTX-M-15 [n= 1], and CTX-M-55 [n= 1]). Of 11 CTX-M-positive strains, four strains were O25b-ST131 clones harboring CTX-M-27, and the remaining seven strains belonged to O6-ST127, ONT-ST354, O159-ST539, O1-ST648, O8-ST1642, O25b-ST2042, and ONT-ST2178. Extraintestinal pathogenic Escherichia coli (ExPEC) are major pathogens that can cause urinary and genital tract infections in dogs and cats, and are characterized by specific virulence factors that promote extraintestinal infection, such as P fimbriae, hemolysin, and cytotoxic necrotizing factor I (1).